ELISA試劑盒煙酰胺腺嘌呤二核苷酸磷酸白色粉末。極易吸潮。能溶于水和甲醇,較少溶于乙醇,幾乎不溶于醚和乙酸乙酯。固體在0℃或室溫干燥品內(nèi)穩(wěn)定。自由酸在冷處幾周內(nèi)穩(wěn)定。在強酸或稀堿溶液中汲輔酶I穩(wěn)定。
應(yīng)用雙抗體夾心法測定標(biāo)本中大鼠煙酰胺腺嘌呤二核苷酸磷酸產(chǎn)物ELISA試劑盒水平。用純化的大鼠煙酰胺腺嘌呤二核苷酸磷酸產(chǎn)物ELISA試劑盒抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入煙酰胺腺嘌呤二核苷酸磷酸產(chǎn)物ELISA試劑盒,再與HRP標(biāo)記的煙酰胺腺嘌呤二核苷酸磷酸產(chǎn)物ELISA試劑盒抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的煙酰胺腺嘌呤二核苷酸磷酸產(chǎn)物ELISA試劑盒呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(OD值),通過標(biāo)準(zhǔn)曲線計算樣品中大鼠煙酰胺腺嘌呤二核苷酸磷酸產(chǎn)物ELISA試劑盒濃度。
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